Composite

Part:BBa_K3763023:Design

Designed by: Guokai Wang   Group: iGEM21_WHU-China   (2021-10-18)


the expression operator in pET26b+ and CDS of PctA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 356
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 340


Design Notes

The reason that we chose His-tag is that small 6xHis tail might not change the ability of our interested proteins so we can not only purify the interested proteins but also directly test its inhibition ability against P. acne.


Source

The T7 promotor,lac operator,RBS 1,6*His tag and T7 terminator come from the plasmid pET26b+ and Sec leader peptide-PctA CDS comes from basic part BBa_3763021.


References